The MinIT is an advanced computational device with the necessary features to co-ordinate basecalling in real-time, via integrated Guppy placed in MinKNOW. Real-time basecalling is governed by the 8 GB RAM for manipulation of sequence data, and the GPU basecalling accelerator drives the basecalling through Lathe generates raw basecalled data using Guppy v.2.3.5 and produces two subassemblies in two separate runs with either Flye v.2.4.2 with the -meta parameter, or Canu v.1.8 using the -nanopore preset parameter 22. In either case, the two runs differ by the estimated genomeSize parameter, provided as 50 and 100 m for Canu, or 100 and 250 m for Flye.
We combined it with 96 #Nanopore PCR barcodes, so guppy deplexes out of the box. We sequenced the same subset of SARS-CoV-2 samples using #Illumina , standard #Nanopore with ligation, and CoronaHiT (48 and 94 samples) with a range of Cts.
Nov 29, 2018 · Flappy is available now from ONT's GitHub site; Guppy should be released by mid-December. Clive also spoke of another approach to improved accuracy which they are calling 8B4. This adds a step to library production in which the native DNA goes through one round of synthesis with all four natural nucleotides but also four base analogs.